21 May 2011

Onion DNA Extraction


    Fresh onions
    Graduated cylinders (10ml and 100ml)
    15 ml test tube
    Test tube rack or 250 ml beaker
    Glass stirring rod
    Coffee filters
    Non-iodized salt
    Adolph's natural meat tenderizer
    Palmolive detergent
    Distilled water
    Ice cold 95% ethanol

Detergent/salt solution:

    20 ml detergent
    20 g non-iodized salt
    180 ml distilled water

5% meat tenderizer solution:

    5 g meat tenderizer
    95 ml distilled water


    Cut an inch square out of the center of 3 medium onions. Chop and place in a blender.

    Add 100 ml of detergent/salt solution.

    Blend on high 30 sec-1 minute.

    Strain the mixture into a beaker using a strainer with a coffee filter.

    Add 20-30 ml meat tenderizer and stir to mix.

    Place 6 ml filtrate in a test tube.

    Pour 6 ml ice cold ethanol carefully down the side of the tube to form a layer.

    Let the mixture sit undisturbed 2-3 minutes until bubbling stops.

    The DNA will float in the alcohol. Swirl a glass stirring rod at the interface of the two layers to see the small threads of DNA.

Modified from: "Isolation of DNA from Onion" Ellen Averill

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