21 May 2011

Onion DNA Extraction

Materials

    Fresh onions
    Graduated cylinders (10ml and 100ml)
    Knife
    15 ml test tube
    Blender
    Test tube rack or 250 ml beaker
    Strainer
    Glass stirring rod
    Coffee filters
    Non-iodized salt
    Adolph's natural meat tenderizer
    Palmolive detergent
    Beaker
    Distilled water
    Ice cold 95% ethanol

Solutions
Detergent/salt solution:

    20 ml detergent
    20 g non-iodized salt
    180 ml distilled water

5% meat tenderizer solution:

    5 g meat tenderizer
    95 ml distilled water

Protocol

    Cut an inch square out of the center of 3 medium onions. Chop and place in a blender.

    Add 100 ml of detergent/salt solution.

    Blend on high 30 sec-1 minute.

    Strain the mixture into a beaker using a strainer with a coffee filter.

    Add 20-30 ml meat tenderizer and stir to mix.

    Place 6 ml filtrate in a test tube.

    Pour 6 ml ice cold ethanol carefully down the side of the tube to form a layer.

    Let the mixture sit undisturbed 2-3 minutes until bubbling stops.

    The DNA will float in the alcohol. Swirl a glass stirring rod at the interface of the two layers to see the small threads of DNA.

Modified from: "Isolation of DNA from Onion" Ellen Averill

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