27 May 2011


                        To predict the gene regions in the given genomic DNA sequence by using a gene prediction tool like genscan.

Genscan server is maintained at MIT provides access to the program genscan for predicting the locations exons and introns structures in genomic sequences from a variety of organisms. The server can accept sequences upto one million base pairs in length. The accuracy of genscanis somewhat sensitive to exon length. Genscan is by far the most comprehensive and sophistication gene prediction tool available for free.

(1) GN.Ex---It indicates gene number, exon number.
(2) INIT---Initial Exon
(3)INTR---Internal Exon
(4)TERN---Terminal Exon
(5)SNGL---Single Exon Gene
(7)PLYA---Poly A Signal
(8)S---DNA strand (+indicates input strand ) (-ve indicates complementary strand)
(9)BEGIN---Beginning position of Exon
(10) END---Ending position of Exon
(11)LEN---Length of Exon
(12)FR--- It indicated Reading Frame
(13)PH--- Net phase of Exon
(14)I-AC--- Initiation signal
(15)DO-T--- Terminal signal score
(16)COD-RG--- Coding region score
(17)P--- Probability of Exons
(18)TSE-R--- Exon’s score {depends on length}

             The score of a predicted feature exon are splice side is a log-odds measure of the quality of a feature based on local sequence properties.

                 The probability of a predicted exon is the estimated probability under genscan’s model. Genomic sequence structure that the exon is correct.

                 We can utilize the file transfer protocol feature of NCBI to obtain complete genome sequences of any of the sequenced organisms freely available. To do this we enter the ftp site of NCBI. It is available at


                   We access the genome directories of all available. A list of organisms
(1)  We enter the ftp site of NCBI.
(2)  From this site we retrieve the complete genome sequence of any organism.
                    (3) We enter into the genscan server at


                    (4) We upload the retrieved genomic sequence and run Genscan.

                    (5) Thus the predicted exons were analysed.


  1. Thanks for such a pleasant post. This post loaded with lots of useful information. Keep it up. If you are looking for the best information and suggestions related to Match Win Prediction then visit Crowdwisdom360 Private Limited

  2. University of Perpetual Help System Dalta Top Medical College in Philippines
    University of Perpetual Help System Dalta (UPHSD), is a co-education Institution of higher learning located in Las Pinas City, Metro Manila, Philippines. founded in 1975 by Dr. (Brigadier) Antonio Tamayo, Dr. Daisy Tamayo, and Ernesto Crisostomo as Perpetual Help College of Rizal (PHCR). Las Pinas near Metro Manila is the main campus. It has nine campuses offering over 70 courses in 20 colleges.

    UV Gullas College of Medicine is one of Top Medical College in Philippines in Cebu city. International students have the opportunity to study medicine in the Philippines at an affordable cost and at world-class universities. The college has successful alumni who have achieved well in the fields of law, business, politics, academe, medicine, sports, and other endeavors. At the University of the Visayas, we prepare students for global competition.

  3. If more people that write articles involved themselves with writing great content like you, more readers would be interested in their writings. I have learned too many things from your article. semrush pricing