29 May 2011


DNA coding

To understand the functional and structural aspects of a gene, lone needs to know the sequence of a gene. Sequencing can be primarily by using two methodologies:

A) Directed Sequencing of BAC Contigs - BAC (Bacterial Artificial Chromosome) vectors are used to get on to genomic libraries in which the slot in size is 80-100 kb. After screening and locating the ordinary restriction fragments, the BAC clones are at that time mapped to discover the overlapping arrays of close clones called contigs. The generous genetic material fragments are at that time kaput into small pieces to complete the sequencing of mapped contigs. Hence,
Using this directed strategy, genetic material pieces from adjacent stretches of a DNA are sequenced.

B) Random Shotgun Sequencing - inside this method, the genome is at random kaput up into sizes of 2-10 kb range and at that time inserted into a vector (plasmids). These are at that time sequenced with the help of overlapping or ordinary sequences. A notebook curriculum identifies the overlapping sequences and joins them into lone unremitting stretch.

A) It provides a basis pro the discovery of all genes and hence lone can construct an supply of genes.
B) The sequence shows the relationships linking the genes which helps in prospect experimentation.
C) The sequencing provides an pointer to magnet and organize all genetic in rank in this area the organism.
D) For era to occur, the total genome sequence becomes an archive pro the prospect which contains all the genetic in rank vital to get on to an organism.
After the sequencing of the genes, we can investigate the functional properties such as the rate of transcript, the level of face under uncommon environmental conditions of genes. Two techniques be inflicted with been used to understand the working of the genes

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