30 May 2011

Basic Theory of Sedimentation

There are many type of preparative centrifugation such as rate zonal, differential, isopycnic centrifugation.

Molecules separate according to their size, appearance, density, viscosity, and centrifugal force. The simplest set of circumstances is a rotund molecule. If the liquid has the density of figure out and the molecule has a density of d and if d>do so therefore the protein will residue. Taking part in gravitational sports ground, the motor force (P) equals the increase in speed of gravity (g) multiplied by the difference flanked by the main part of the molecule and the main part of a corresponding volume of form.

What happens to a particle (macromolecule) in a centrifugal sports ground?

Consider a particle m in a centrifuge tube crammed with a liquid.

The particle (m) is acted on by three forces:

FC: The centrifugal force

FB: The buoyant force

Ff: The frictional force flanked by the particle and the liquid

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