29 May 2011

FLUORESECENT IN SITU HYBRIDISATION (FISH)


The reporter fluorescent molecule is tagged on the cDNA molecule by using the notch translation practice urban by Rigby and Paul Berg in 1977. Inside this method, the enzyme DNase I cuts genetic material and genetic material polymerase I makes genetic material. These two are combined in a result along with dNTPs with the dUTP labeled with a red or conservational fluorescence. The genetic material pol I adds dNMP’s to the 3’OH last stop in the nick with the intention of has already been produced by DNases so incorporating the genetic material with fluorescent nucleotides. This practice renowned as Fluorescence in situ Hybridization (FISH) allows knowing the status in the interphase unlike in karyotyping everywhere you need a metaphase DNA. The attention of FISH practice was illustrated by taking the model of chronic mylogenous leukemia (CML) in which the karyotype analysis revealed with the intention of here was a   9-22 translocation in the DNA (Philadelphia chromosome)

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