25 May 2011

High-Performance Liquid Chromatography (HPLC)

High-performance liquid chromatography (HPLC) is a form of liquid chromatography to separate compounds with the intention of are dissolved in solution. HPLC instruments consist of a reservoir of mobile period, a pump, an injector, a separation column, and a detector. Compounds are separated by injecting a plug of the sample mixture on the column. The uncommon components in the mixture pass through the column by uncommon tariff due to differences in their partitioning behavior linking the mobile liquid period and the stationary phase.

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