25 Jun 2011

Chromatography


Introduction
Chromatography is a separations method with the intention of relies on differences in partitioning behavior linking a flowing mobile period and a stationary period to separate the the components in a mixture.
A discourse (or other support pro TLC, think it over below) holds the stationary period and the mobile period carries the sample through it. Sample components with the intention of partition strongly into the stationary period waste a greater amount of calculate in the discourse and are separated from components with the intention of stay predominantly in the mobile period and pass through the discourse nearer.
Equally the components elute from the discourse they can be quantified by a detector and/or collected pro additional analysis. An analytical instrument can be combined with a separation method pro on-line analysis. Examples of such "hyphenated techniques" include chatter and liquid chromatography with bulk spectrometry (GC-MS and LC-MS), Fourier-transform infrared spectroscopy (GC-FTIR), and diode-array UV-VIS captivation spectroscopy (HPLC-UV-VIS).

Specific chromatographic methods:

    Applied to precarious organic compounds. The mobile phase is chatter (gas) and the stationary phase is ordinarily a liquid on a solid support or now and again a solid adsorbent.

    A variation of liquid chromatography by the intention of utilizes high-pressure pumps to increase the efficiency of the separation.

    Used to separate analyses in solution counting metal ions and organic compounds. The mobile period is a solvent and the stationary phase is a liquid on a solid support, a solid, or an ion-exchange resin.

    Also called gel-permeation chromatography (GPC), the mobile period is a solvent and the stationary period is a packing of porous particles.

    A simple and rapid method to watch the boundary of a result or to check the purity of organic compounds. The mobile period is a solvent and the stationary period is a solid adsorbent on a flat tire support.



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