4 Dec 2011

Extraction of genetic material (DNA) from creature cheek cells - Presentation Transcript

1. Extraction of genetic material from Human Cheek Cells

2. Extracting genetic material from Your Cells
Today you will extract genetic material from your cheek cells. This process is akin to what is made as one DNA-containing tissue is found by a crime scene.
Cells from the lining of your mouth come loose easily, so you will be able to assemble cells containing your genetic material by swishing a liquid around in your backtalk. The cells from the lining of your backtalk furthermore occur rancid when you chew food.

3. Extracting genetic material from Your Cells
To extract genetic material from your cells, you will need to separate the genetic material from the other types of biological molecules in your cells. What are the other foremost types of generous biological molecules in cells?

4. Extracting genetic material from Your Cells
You will be using the same basic steps with the intention of biologists aid as they extract genetic material (e.G. To clone genetic material or to get on to a genetic material fingerprint). You will stay on these 3 straightforward steps to extract the genetic material:
Detergent
ENzymes (meat tenderizer)
Alcohol

5. Getting Your Sample of Cells
Obtain a cup with sports drink. You will need to make thousands of your cheek cells in the sports drink in order to extract sufficient genetic material to think it over.
Swish the sports drink around in your backtalk vigorously pro by smallest amount lone little.
Chew on your cheeks while swishing so you make as many cells as doable.
Spit the drink back into the cup
Repeat a hardly any era

6. Step 1: Detergent
Add a small amount of detergent to a test tube (about 0.25 mL).
Now carefully pour the drink containing your cheek cells
Into the test tube with
Detergent until the tube is
Semi satiated.

7. Why am I count detergent?
To make the genetic material made known of your cheek cells you need to break commence both the cell membranes and the nuclear membranes. Cell membranes and nuclear membranes consist primarily of lipids. Dishwashing detergent, like all soaps, breaks up lipids. This is why you aid detergents to remove fats (which are lipids) from soil dishes! Adding the detergent to you cheek cell solution will break commence the cell membranes and nuclear membranes and relief your genetic material into the solution.

8. Step 2: Enzymes
Add a pinch of enzyme (meat tenderizer) to your test tube.
Put the cover on the test tube; gently invert the tube five era to mix.
Let the mixture sit pro by smallest amount 10 minutes.
Break HERE!

9. Why am I count enzymes?
The heart of all of your cells contains multiple long strands of genetic material with all the directions to get on to your full body. If you stretched made known the genetic material found in lone of your cells, it would be 2-3 meters long. To fit all of this genetic material inside a tiny cell heart, the genetic material is wrapped tightly around proteins. The enzyme in meat tenderizer is a protease, which is an enzyme with the intention of cuts proteins into small pieces. Equally this enzyme cuts up the proteins, the genetic material will untwist and separate from the proteins.

10. Step 3: Alcohol
Using a pipette, unhurriedly add cold alcohol
Into the test tube;
Consent to the alcohol run down the feature of the test
Tube so it forms a layer on top of the bubbly
Liquid.
Add alcohol until you be inflicted with in this area 2 cm of alcohol in the tube.
Alcohol is a reduced amount of dense than fill up, so it floats on top. Do not mix or bump the test tube pro 10 minutes.

11. Why am I count alcohol?
The cold alcohol reduces the solubility of genetic material. When cold alcohol is poured on top of the solution, the genetic material precipitates made known into the alcohol layer, while the lipids and proteins stay in the solution. Genetic material molecules will plod collectively everywhere the bubbly fill up not more than meets the cold alcohol higher than, and you will be able to think it over these clumps of genetic material as white strands.

12. Steps pro genetic material Extraction


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